Abcam Ad

Author Topic: Antigen retrieval questions  (Read 5160 times)

0 Members and 1 Guest are viewing this topic.

Offline qtian

  • Newbie
  • *
  • Posts: 4
Antigen retrieval questions
« on: April 29, 2003, 01:09:23 PM »
:?: I am new about antigen retrieval. Can anyone give me some basic information about this technique. At what circumstances, I should use or not use antigen retieval technique.

Thank you in advance for your help.

Tina

Antigen retrieval questions
« on: April 29, 2003, 01:09:23 PM »

Offline brian

  • HighTech
  • ***
  • Posts: 17
Antigen retrieval questions
« Reply #1 on: May 06, 2003, 08:39:36 PM »
Antigen retrieval technique is mainly used for formalin-fixed, paraffin-embedded tissue sections. You can go to this link http://www.ihcworld.com/introduction.htm#antigenretrieval for basic information about this technique. If you are working with paraffin sections a lot, I recommend you to read a book called: "Antigen Retrieval Techniques: Immunohistochemistry and Molecular Morphology". Check this link http://www.ihcworld.com/bookstore.htm for the book information.

Brian

Offline peter

  • Trainee
  • **
  • Posts: 5
Antigen retrieval questions
« Reply #2 on: July 05, 2003, 03:42:07 PM »
When you do double labeling, the combination of HIER and PIER will be very useful as well.

Peter

Antigen retrieval questions
« Reply #2 on: July 05, 2003, 03:42:07 PM »