Author Topic: myosin heavy chain antigen retrieval and ALD-58  (Read 8300 times)

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Offline MikeJorg

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myosin heavy chain antigen retrieval and ALD-58
« on: December 23, 2009, 04:16:54 PM »
I am using ALD-58 in IHC to detect slow-tonic skeletal muscle fibers in sections of amphibian muscles.  Is it possible to do this with formalin-fixed, alcohol preserved museum specimens using retrieval techniques?  Any feedback would be great because I am fairly new to IHC and its uses.  Thanks!

-Mike

Mike Jorgensen
PhD Candidate
Department of Biological Sciences
Ohio University
Athens, Ohio 45701
Email: <mj207406@ohio.edu>

myosin heavy chain antigen retrieval and ALD-58
« on: December 23, 2009, 04:16:54 PM »

Offline excalibur

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Re: myosin heavy chain antigen retrieval and ALD-58
« Reply #1 on: December 27, 2009, 11:18:06 AM »
All you can do is try, but they may have been fixed for too long a period of time.
Paula K. Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
8901 S Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3959
www.excaliburpathology.com

Offline Bastet13

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Re: myosin heavy chain antigen retrieval and ALD-58
« Reply #2 on: February 15, 2010, 12:52:57 PM »
Hi,

I have found that using a deparaffinization of 30 mins x 3 (or more!) in xylene substitute can really make a difference in exposing antigen sites.  That is the technique I use, in addition to varying types of antigen retrieval, when tissues have been in formalin for a long time.

Good luck!

Sara

Offline sunshine_gxl

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Re: myosin heavy chain antigen retrieval and ALD-58
« Reply #3 on: September 20, 2010, 11:41:46 PM »
I am using ALD-58 in IHC to detect slow-tonic skeletal muscle fibers in sections of amphibian muscles.  Is it possible to do this with formalin-fixed, alcohol preserved museum specimens using retrieval techniques?  Any feedback would be great because I am fairly new to IHC and its uses.  Thanks!

-Mike

Mike Jorgensen
PhD Candidate
Department of Biological Sciences
Ohio University
Athens, Ohio 45701
Email: <mj207406@ohio.edu>
Have you tried the double antigen retrieval?

Re: myosin heavy chain antigen retrieval and ALD-58
« Reply #3 on: September 20, 2010, 11:41:46 PM »