Abcam Ad

Author Topic: CD15 ON VENTANA BENCHMARK XT  (Read 4700 times)

0 Members and 1 Guest are viewing this topic.

Offline fresnotech

  • Newbie
  • *
  • Posts: 3
CD15 ON VENTANA BENCHMARK XT
« on: December 29, 2009, 07:47:02 PM »
HAS ANYONE HAD PROBLEMS WITH THE VENTANA CD15 ANTIBODY ON THE BENCHMARK XT DEMONSTRATING REED STERNBERG CELLS?
IN OUR LAB, ONLY THE NEUTROPHILS STAIN ON OUR HODKINS CONTROL SLIDE. CAN YOU SUGGEST A PROTOCOL THAT WOULD ENABLE US TO OBTAIN THE EXPECTED RESULTS ACCORDING TO THE VENTANA DATASHEET - >CC1, >AMPLIFICATION, >ANTIBODY TIME ETC. ALSO WILLING TO TRY ANOTHER SUPPLIER -THE CD15 ANTIBODY FROM CELLMARQUE LOOKS PROMISING AND IS AVAILABLE IN VENTANA DISPENSERS. BEFORE AUTOMATION GREAT RESULTS WERE POSSIBLE USING A DEDICATED IGM SECONDARY ANTIBODY BUT OUR LAB IS GLUED TO VENTANA DETECTION KITS, UNDERSTANDABLY. I AM GRATEFUL FOR ANY SUGGESTIONS. nmason28@hotmail.com

CD15 ON VENTANA BENCHMARK XT
« on: December 29, 2009, 07:47:02 PM »