Abcam Ad

Author Topic: Antigenic retrieval B4 IHC of matrix proteins?  (Read 4764 times)

0 Members and 1 Guest are viewing this topic.

Offline cherrypicka

  • Newbie
  • *
  • Posts: 3
Antigenic retrieval B4 IHC of matrix proteins?
« on: May 27, 2003, 12:16:05 PM »
Ello Everyone,

I have tried trypsin for IHC with fairly good results with collagen I, Elastin, Fibronectin and Vitronectin.

I am wondering if, say Trypsin is good for exposing some ECM proteins while Pepsin, Papain or others is good for exposing other Extracellular matrix proteins. Does anyone use protease digestion for IHC of matrix proteins and which is their favourite enzyme.

Alternatively does anyone prefer heat induced antigenic retrieval?

Any immunohisto folklore with regard to matrix proteins would be brilliant, in fact!

Yours Hopefully!

Gail
ch liebe Rammstein

Antigenic retrieval B4 IHC of matrix proteins?
« on: May 27, 2003, 12:16:05 PM »

Offline peter

  • Trainee
  • **
  • Posts: 5
Antigenic retrieval B4 IHC of matrix proteins?
« Reply #1 on: May 27, 2003, 04:48:59 PM »
If an enzyme digestion (i.e. trypsin) works well with a protein, it usually will work well with similar proteins. I like to use trypsin for most of my IHC applications and it works very well.

Heat induced antigen retrieval is usually working better for the recovery of nuclear proteins, such as p53, p63, ki67, etc.

You should always test different antigen retrieval conditions for a new antibody.

Peter

Antigenic retrieval B4 IHC of matrix proteins?
« Reply #1 on: May 27, 2003, 04:48:59 PM »