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Neuropathological diagnosis of age related diseases

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Author Topic: IHC for age related cytoskeletal diseases  (Read 2493 times)

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Offline Abhinash

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IHC for age related cytoskeletal diseases
« on: March 10, 2010, 12:43:39 AM »
1) I have collected ~70 autopsy human brains above 50yrs includes hippocampus, S.Nigra, M.Tem.Gyrus, Caudate Nucleus, White matter, S.Parie. Lobule and S.Front. Gyrus

2) I have to look for age related cytoskeletal pathologies using IHC

3) I plan to use beta-amyloid, Tau and alpha synuclien to look those features mainly AD, PD, DLB, FTD, PSP, CBD, MSA, HD, Pick disease and ALS.

4) Please advise me and share your experiences regarding these neuropathological diagnosis and please advise if you have seen any new prrotocls or antibody kits..

IHC for age related cytoskeletal diseases
« on: March 10, 2010, 12:43:39 AM »

Offline funk.106

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Re: IHC for age related cytoskeletal diseases
« Reply #1 on: March 10, 2010, 11:24:07 AM »
You may want to check out this paper using p62 as a diagnostic:
E. Kuusisto, T. Kauppinen and I. Alafuzoff (2008) Neuropathology and Applied Neurobiology 34, 169180
Use of p62/SQSTM1 antibodies for neuropathological diagnosis

I use the pAb from enzo (biomol) with great results for staining the neurofibrillary tangles. Also for tau staining, I use Tau5, however you should know that at least for me, it required a second step of antigen retrieval. Following 20 minutes in the microwave with citrate buffer, I do a 5 minute incubation with a proteinase k solution (you can find protocols for enzymatic digestion...im sure any of them will work).

Re: IHC for age related cytoskeletal diseases
« Reply #1 on: March 10, 2010, 11:24:07 AM »