Author Topic: protocol for mouse mammary gland Ag retrieval  (Read 6546 times)

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Offline lemonde79

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protocol for mouse mammary gland Ag retrieval
« on: March 10, 2010, 05:03:00 PM »
Hello. I am trying to do some IHC staining in mouse mammary gland. Could somebody tell me the best way to do Ag retrieval of mammary gland? Thank you so much.

protocol for mouse mammary gland Ag retrieval
« on: March 10, 2010, 05:03:00 PM »

Offline excalibur

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Re: protocol for mouse mammary gland Ag retrieval
« Reply #1 on: April 09, 2010, 01:05:27 PM »
The antibody used is dependent on the retrieval method, not the tissue.

What does the datasheet for your antibody state?
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Offline richard03

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Re: protocol for mouse mammary gland Ag retrieval
« Reply #2 on: April 18, 2010, 01:33:34 PM »
If the datasheet does not state which Antigen Retrieval method to use, you may select one or few methods from the list on this page and have them tested.

http://www.ihcworld.com/epitope_retrieval.htm

Re: protocol for mouse mammary gland Ag retrieval
« Reply #2 on: April 18, 2010, 01:33:34 PM »