Abcam Ad

Author Topic: tissue detachment  (Read 4996 times)

0 Members and 1 Guest are viewing this topic.

Offline labgal

  • Trainee
  • **
  • Posts: 8
tissue detachment
« on: March 11, 2010, 04:17:07 PM »
I am having a problem with needle core biopsy tissue comming off the slides. I am currently using super frost plus slides with a little gelatin in the water, the paraffin is sections at 3 microns then air dried standing up and left on a slide warmer flat over night, they are then placed in a 55 degree oven for 1 hour before staining. The sections are stained on an autostainer so manipulation of the tissue should be at a minimum. Does anyone have any idea's as to why some of the tissue is detaching? The slides are a new batch so the charge should be good.

tissue detachment
« on: March 11, 2010, 04:17:07 PM »

Offline excalibur

  • Global Moderator
  • GoldMember
  • *****
  • Posts: 1109
    • http://www.excaliburpathology.com
Re: tissue detachment
« Reply #1 on: March 12, 2010, 09:39:34 AM »
Try skipping the slide warmer and leave them in the oven overnight instead.

How many xylenes to deparaffinize? How long in each? Are they and the alcohols clean? Especially the last ones.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline richard03

  • Administrator
  • GoldMember
  • *****
  • Posts: 1212
Re: tissue detachment
« Reply #2 on: April 18, 2010, 12:13:17 PM »
I found putting enough gelatin (i.e. >=0.03% concentration) in water bath will help tissue sections stay on slides. But the potential drawback is higher background staining may occur.

Offline Yao

  • HighTech
  • ***
  • Posts: 15
Re: tissue detachment
« Reply #3 on: June 04, 2010, 05:02:37 AM »
maybe the slides are too good. we use common APES or polylysine coated slides, work well, no detachment. but, super frost slides are superior for frozen section.

Offline gula

  • Global Moderator
  • GoldMember
  • *****
  • Posts: 356
Re: tissue detachment
« Reply #4 on: June 04, 2010, 01:18:01 PM »
Do you notice trapped water under the paraffin slides after the first drying?
Are the superfrost plus slides beyond shelf life? Are they stored open in warm environment?
Would it work better without gelatine?
gula

Re: tissue detachment
« Reply #4 on: June 04, 2010, 01:18:01 PM »