I'm currently working on the effects of kainate (an excitotoxicity agent) on dysbindin ( a schizophrenia susceptibility gene)
The results that i've gotten for western blot and immunohistochemistry results differs.
For western blot, I've harvested the hippocampus at the site of injury and results showed a significant upregulation as compared to the controls. This information is in tune with my real-time pcr results.
However, my IHC results have shown a downregulation at the site of injury (right hippocampus) and a significant upregulation at site away from the site of injury (left hippocampus).
As such, I am at a lost and have no idea how I should write my thesis which is due this month... I really need as much advice as possible.
Thank you for your help Cheesy