Author Topic: DNA in sample  (Read 3689 times)

0 Members and 1 Guest are viewing this topic.

Offline Neurochica

  • Newbie
  • *
  • Posts: 2
DNA in sample
« on: May 25, 2010, 03:46:47 PM »
Hi everyone,

Full story: I recently prepped some tissue for western blot and noticed when I was loading my gel that there was still a fair amount of DNA in my prepped tissue sample. I ran the gel and got bands, a few where I expected, and some that I did not quite expect. At the moment I am trying to troubleshoot what some of these bands are or why they may be present. One possible source of the bands on my blot is the DNA in my sample (I believe...??), but I have had lots of trouble figuring out exactly what DNA would look like on a western blot. Could the DNA be causing non-specific bands? What should I expect my blot to look like with a decent amount of DNA in my sample??

Short and sweet: What does DNA look like on a western blot?

Thanks for the help!!

DNA in sample
« on: May 25, 2010, 03:46:47 PM »