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Author Topic: AB dilema  (Read 2661 times)

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Offline Jason

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AB dilema
« on: June 18, 2003, 10:43:13 PM »
I am woking on a protein which shows higher levels of expression in tumor tissue than in normal tissue on western blots. When I did IHC on For. Fixed-Par. Embedded Normal and Tumor tissue, they both stained positive. I lowered the concentration of 1' Ab, but I lose the signal in the tumor tissue first. Can this be right?

Thanks all

AB dilema
« on: June 18, 2003, 10:43:13 PM »

Offline philtrougouboff

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AB dilema
« Reply #1 on: June 19, 2003, 03:25:12 AM »
:?:
Hi Jason,
When you write that both nl. tissue and tumor stained positive by IHC, do you mean strictly the same way or is there  differences in intensity/cell, localisation of the Ab, number of positive cells in each one, etc  :?:
(must be nice to be in San Diego)
hilippe Trougouboff, MD

Offline Jason

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AB dilema
« Reply #2 on: June 25, 2003, 04:37:15 PM »
The intensity seems to be the same at higher concentrations, but as I lower the concentrations, the intensity deminishes.  Unfortunately, our normal tissue is retaining the DAB signal longer.  The percentage of cells stained is virtually the same.

Jason :oops:

AB dilema
« Reply #2 on: June 25, 2003, 04:37:15 PM »