Author Topic: Mitotracker in cultered cells  (Read 4496 times)

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Offline Sanaz

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Mitotracker in cultered cells
« on: August 06, 2010, 02:07:19 AM »
HI All,
          I am looking for advice on why the mitotracker red dye my student is using keeps localisaing in the nucleus , yet, only leaves a very wishy washy cytoplasmic blush, and not the strong punctiform staining. The protocol has been used to the letter and fresh batches of 4% paraformaldehyde, or even ice cold acetone always used. They are transfected HEK293 cells. Thanks so much.

Mitotracker in cultered cells
« on: August 06, 2010, 02:07:19 AM »