Sorry to bump this topic, but I was looking for some advice with this kit. I'm having difficulty getting a positive signal with my DNAseI control. I don't think the DNAseI treatment is the problem itself, I believe the issue is with the fixation and permeabilization conditions I use.
I am performing my experiments on HeLa cells, using the conditions described in the handbook (2% Paraformaldehyde in PBS, 1 hour; followed by 0.1% Triton X-100 in 0.1% Na-citrate for 2 mins on ice), but have also tried 2% PFA in PBS, 1 hour; followed by 0.5% Triton X-100 in 0.1% Na-citrate for 10 mins at room temperature with no success.
Has anyone had successful experiments with this kit on HeLa cells? I can obviously try lots of different conditions but was hoping for some time-saving advice!