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Author Topic: Dilutions  (Read 4412 times)

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Offline mmerrell

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Dilutions
« on: July 09, 2003, 04:21:49 PM »
Hi!  I am doing a set of slides next week with two separate antibodies with identical protocols, except for one thing.  One antigen retrieval uses 0.1mM citrate, while the other requires 10mM.  My question is, does the dilution factor make that much of a difference, or will it just depend on the individual antibody?  One of these antibodies I have used before, and one I have not.
Thanks!
Melinda

Dilutions
« on: July 09, 2003, 04:21:49 PM »

Offline peter

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Dilutions
« Reply #1 on: July 09, 2003, 09:05:36 PM »
Melinda,

I always use 10mM (0.01M) citrate buffer (pH6.0) and works very well. But haven't seen protocols using 1mM (0.001M) citrate buffer for antigen retrieval purpose. I am very curious to know where you get the "1mM citrate buffer" protocol from? Have you been using this successfully?

I think (just a guess) pH value is more important factor to be considered than concentration for HIER. Be glad to hear other comments as well.

Peter.

Dilutions
« Reply #1 on: July 09, 2003, 09:05:36 PM »