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Author Topic: Dopamine 1 receptor antibody  (Read 3711 times)

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Offline catia

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Dopamine 1 receptor antibody
« on: August 31, 2010, 08:56:19 AM »
 I am facing some difficulties to detect D1R by western-blotting in plasma membrane fractions from renal cortex. I have used 2 primary antibodies (from Alpha Diagnostics and Chemicon) and tried several dilutions but none as functioned. I load 8-10Ķg of protein and I canít visualize any band in the membrane. Does anybody have any suggestion regarding the use of these antibodies in plasma membrane fraction? I follow a standard protocol but could it be necessary to do any additional step in the case of membrane fractions?

Dopamine 1 receptor antibody
« on: August 31, 2010, 08:56:19 AM »

Offline SGlerup

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Re: Dopamine 1 receptor antibody
« Reply #1 on: January 05, 2013, 11:20:50 AM »
Hi Catia
We use anti DRD1 Polyclonal antibody from Proteintech with the catalog number
17934-1-AP.

It is really good for Western blotting.
We have reviewed it on www.pabmabs.com
Simon

Re: Dopamine 1 receptor antibody
« Reply #1 on: January 05, 2013, 11:20:50 AM »