Abcam Ad

Author Topic: How to Elute Plasmid I just received  (Read 2820 times)

0 Members and 1 Guest are viewing this topic.

Offline Montisumo

  • HighTech
  • ***
  • Posts: 13
How to Elute Plasmid I just received
« on: September 08, 2010, 11:50:53 AM »
Hi,

I just received an expression plasmid sent to me via snail mail. The senders said they spotted 5 micrograms DNA in each of 2 circles on TLC paper. They suggest I cut out a circle and elute in 100 microliters of TE then transform cells.

1. Does this mean I just cut the circle they spotted (containing the 5 ug of plasmid) then add to 100ul TE buffer???
2. Will the TLC paper dissolve by itself?
3. How long will the expression plasmid be stable on TLC paper stored @ 4C?
4. How long will it be stable once eluted in TE buffer @ 4C?

Thanks!

How to Elute Plasmid I just received
« on: September 08, 2010, 11:50:53 AM »