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Author Topic: apoptosis or necrosis or...?  (Read 10560 times)

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Offline angela

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apoptosis or necrosis or...?
« on: July 18, 2003, 09:27:47 PM »
Hi.
I'm new in the forum, so I don't know if I am in the right one. I'm working with mouse brain section, and I need to see which kind of death the missing Purkinje cells die of....
I tried a kit to see if there is any apoptosis and also an immunostaining with anti-caspase3, with no result. I'm wondering if there are others markers for necrosis or other kinds of neuronal death I can try on paraffin sections.
I will apreciate any suggestion.

thanks

angela

apoptosis or necrosis or...?
« on: July 18, 2003, 09:27:47 PM »

Offline richard03

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apoptosis or necrosis or...?
« Reply #1 on: July 20, 2003, 11:19:55 PM »
Angela,

Apoptotic cell death, reported to be non-inflammatory, and necrotic cell death, which may be inflammatory, are two extremes, while the real situation is usually more complex and it is often co-exist in the same cell. You may find the following websites helpful for your questions.
http://users.rcn.com/jkimball.ma.ultranet/BiologyPages/A/Apoptosis.html
http://www.neuro.wustl.edu/neuromuscular/mother/apoptosis.htm

As for apoptosis detection, the following methods/markers are often used:
TUNEL, caspases, Annexin V, Bcl-2, Fas, PARP. However, you should always include a POSITIVE CONTROL when you run apoptosis and caspase-3 staining to be sure the procedure is working.

Hope this helps,

Richard

apoptosis or necrosis or...?
« Reply #1 on: July 20, 2003, 11:19:55 PM »