Hello all, I have a prelim coming up where I need to present on methods out of my jurisdiction.
I would like to take samples of primary tumor and seek out the different variants of two well-characterized receptors that are found in them.
Can I take a tissue lysate, purify for my protein of interest and run a mass spec for this? Will this tell me the different variations that are present in the tumor?
Any advice or direction is appreciated, cheers.