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Author Topic: What to do with deparaffinized slides  (Read 5817 times)

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Offline akaandykay

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What to do with deparaffinized slides
« on: October 13, 2010, 07:47:44 PM »
I'm feeling a little dumb. While staining for the MGMT antibody, I put my slides into the oven, then went through the xylenes to deparaffinize, then graded alcohols to rehydrate the tissue, then put them into a pressure cooker to do antigen retrieval, and then let them sit for 30 minutes to cool down. 

It was at this point that I realized I did not have a positive control slide included.  Since my PI always wants to have a positive control slide (a tissue that is a known positive for MGMT) with each staining run, I know that this slide run will be useless and I will need to start all over tomorrow. 

My question is, do I need to use all-new slides, or is there some way I could preserve them until tomorrow when I have a positive control slide ready to be included with them?  I think right now we are going to store the slides in TBS-Tween overnight. 


What to do with deparaffinized slides
« on: October 13, 2010, 07:47:44 PM »

Offline excalibur

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Re: What to do with deparaffinized slides
« Reply #1 on: October 14, 2010, 10:33:08 AM »
You should always use a postive control for any stain you do and the slides will be fine in PBS or TBS overnight at 4C.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
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Re: What to do with deparaffinized slides
« Reply #1 on: October 14, 2010, 10:33:08 AM »