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Author Topic: Carnoy fixative  (Read 5613 times)

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Offline weeza42

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Carnoy fixative
« on: October 14, 2010, 04:40:53 AM »
Hi,

For methanol-carnoy fixative is ethanol substituted by methanol v/v?
Ethanol 60ml
Chloroform 30ml
Glacial acetic acid 10ml

Also, are there any special steps required for the tissue processing through to paraffin embedding or is it normal 70% ethanol, 95% ethanol, 100% ethanol, xylene, paraffin?

thanks Weeza42

Carnoy fixative
« on: October 14, 2010, 04:40:53 AM »

Offline excalibur

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Re: Carnoy fixative
« Reply #1 on: October 14, 2010, 10:35:53 AM »
If you have no methanol, you can substitute EtOH. Process as usual.

FYI: If the processor is also used for formalin fixed tissue, the first alcohols will contain a small amount of formalin from simple carryover.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Offline amosbrooks

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Re: Carnoy fixative
« Reply #2 on: October 26, 2010, 07:00:02 PM »
Hi,
     Carnoy fixative is an anhydrous solution. Once the tissue is properly fixed, it is also dehydrated. To make sure, following the fixation you can place the tissue in 100% methanol (or ethanol), then you can go right into xylene. Why would you take tissue you are attempting to dehydrate in processing and put it back into 95%? Save yourself some time and effort and start from 100%.

Good luck,
Amos

Re: Carnoy fixative
« Reply #2 on: October 26, 2010, 07:00:02 PM »