Author Topic: New to me  (Read 4823 times)

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Offline cincanada

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New to me
« on: November 03, 2010, 01:48:45 PM »
So I have never done this type of immuno before.  We want to stain cells and blood vessels.  Do I need to fix these?  I have worked strictly with fixed tissue in the past (PFA/Sucrose).

Please advise the best way to preserve these and follow through with Immuno.

C

New to me
« on: November 03, 2010, 01:48:45 PM »

Offline MT Scientist

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Re: New to me
« Reply #1 on: November 03, 2010, 02:08:47 PM »
Need more information.....

Offline Cardio

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Re: New to me
« Reply #2 on: November 03, 2010, 04:51:32 PM »
So I have never done this type of immuno before.  We want to stain cells and blood vessels.  Do I need to fix these?  I have worked strictly with fixed tissue in the past (PFA/Sucrose).

Please advise the best way to preserve these and follow through with Immuno.

C

Fixation normally depends on your antibody. You can find out what fixation to use on the antibody datasheet. Normally they have a protocol section. IF they don't then call the company for more information.

Re: New to me
« Reply #2 on: November 03, 2010, 04:51:32 PM »