Methods and Techniques Discussion > Immunocytochemistry (ICC)

New to me

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cincanada:
So I have never done this type of immuno before.  We want to stain cells and blood vessels.  Do I need to fix these?  I have worked strictly with fixed tissue in the past (PFA/Sucrose).

Please advise the best way to preserve these and follow through with Immuno.

C

MT Scientist:
Need more information.....

Cardio:

--- Quote from: cincanada on November 03, 2010, 01:48:45 PM ---So I have never done this type of immuno before.  We want to stain cells and blood vessels.  Do I need to fix these?  I have worked strictly with fixed tissue in the past (PFA/Sucrose).

Please advise the best way to preserve these and follow through with Immuno.

C

--- End quote ---

Fixation normally depends on your antibody. You can find out what fixation to use on the antibody datasheet. Normally they have a protocol section. IF they don't then call the company for more information.

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