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Author Topic: What secondary antibody shall I use?  (Read 1785 times)

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Offline HanneS

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What secondary antibody shall I use?
« on: November 24, 2010, 04:31:47 AM »
I have bought a monoclonal antibody (mouse anti rat-CD68 conjugated to Biotin). I wonder what kind of secondary antibody shall I use? I normally use ABC system from Santa Cruz for developing the slides.
 
Grateful for advice



What secondary antibody shall I use?
« on: November 24, 2010, 04:31:47 AM »

Offline richard03

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Re: What secondary antibody shall I use?
« Reply #1 on: November 24, 2010, 01:00:58 PM »
Since the antibody is conjugated to Biotin so you may not need secondary antibody. You can apply ABC mix or HRP-streptavidin directly after primary antibody incubation.

Re: What secondary antibody shall I use?
« Reply #1 on: November 24, 2010, 01:00:58 PM »