I wanted to share the results of a recent study that was released by The Science Advisory Board on tissue microarrays (TMAs).
The study summary can be viewed at http://www.scienceboard.net/studies/studies.87.html
Also, there's a MP3 file available of a conversation with an expert on tissue microarrays that reviews the key findings from this study. It can be downloaded at http://www.scienceboard.net/snapshotslive/ttp://www.scienceboard.net/snapshotslive/
Although TMAs offer many advantages compared to individual tissue sections, they also present a number of drawbacks. Based on our study, the most serious problem with tissue microarrays is the detachment of specimens during processing. Slight damage or tissue loss during processing usually is not a critical issue with standard tissue sections as long as the majority of the specimen remains intact. With TMAs, however, even minor damage can result in the complete loss of multiple specimens. Therefore, it is very important to include multiple replicates of every specimen during TMA production. Has anyone on this forum begun to address this problem?