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Author Topic: Antigen Retrival Optimization  (Read 7911 times)

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Offline alight03

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Antigen Retrival Optimization
« on: July 28, 2003, 10:31:01 AM »
Hi,

 I am relatively new to antigen retrieval and immunohistochemistry. I am trying to improve upon a current protocol used by my group.  I am interested in information about different antigen retrieval buffers, antigen retrieval methods and wash buffers used for immunohistochemistry of paraffin embeeded slides. Currently, the wash buffer I use is Tris-buffered saline, pH 7.4. In some cases phosphate-buffered saline, ph 7.4,  is also used. As for antigen retrieval, a microwave method is used, with either 10mM sodium citrate buffer, 1mM EDTA or 10mM Tris buffer.   I are was wondering of anyone could share his/her techniques with me. Thank you very much.
ngela

Antigen Retrival Optimization
« on: July 28, 2003, 10:31:01 AM »

Offline susan

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Antigen Retrival Optimization
« Reply #1 on: July 28, 2003, 03:44:12 PM »
Hope the following web pages will give you a little help for your questions

http://www.ihcworld.com/technical_tips.htm

http://www.ihcworld.com/protocol_database.htm#histo

Offline AndrewVF

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Re: Antigen Retrival Optimization
« Reply #2 on: August 18, 2003, 09:02:14 PM »
Hi
Check http://www.biogenex.com/biogenex.html and http://www.innogenex.com. They have several a/g retrieval methods and 6-7 solutions.
The best wash buffer for me is the Tris Buffered Saline, with Tween 20, pH adjusted to 7.4 - 8.0 (Sigma T9039)
Good luck

Andrew

Quote from: "alight03"
Hi,

 I am relatively new to antigen retrieval and immunohistochemistry. I am trying to improve upon a current protocol used by my group.  I am interested in information about different antigen retrieval buffers, antigen retrieval methods and wash buffers used for immunohistochemistry of paraffin embeeded slides. Currently, the wash buffer I use is Tris-buffered saline, pH 7.4. In some cases phosphate-buffered saline, ph 7.4,  is also used. As for antigen retrieval, a microwave method is used, with either 10mM sodium citrate buffer, 1mM EDTA or 10mM Tris buffer.   I are was wondering of anyone could share his/her techniques with me. Thank you very much.
ndrew F., M.D., Ph.D.
Vaccine Research Center
Emory University

Re: Antigen Retrival Optimization
« Reply #2 on: August 18, 2003, 09:02:14 PM »