Hi, so I've got a basic question about western blotting. For the most part, I block my western blot in 4% nonfat milk, and then dilute both antibodies in that blocking buffer and have success. I recently purchased a new antibody, however, for which the data sheet says not to use milk as my blocking agent--to use BSA instead. So I tried to perform a blot--blocked with 5% BSA in TBST and then diluted both primary (Rabbit) and secondary (Goat anti-rabbit) antibodies in 1% BSA-TBST (no sodium azide in either dilution)--and I got nothing. I'm pretty confident that the actual blot worked (markers look good), but I am wondering if I was right to dilute my antibodies in 1% BSA...particularly the secondary? What do you dilute your antibodies in in such a situation? Is there anything I need to be particularly careful about?