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Antigen retrieval in free-floating sections

Antigen retrieval in free-floating
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Author Topic: blue 42  (Read 5166 times)

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Offline hrivera00

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blue 42
« on: January 20, 2011, 05:23:33 PM »
I keep on reading about antigen retrieval conducted with sodium citrate prewarmed in water baths @ 80 degrees Celsius using free-floating tissue?  How is it possible to separate treatment groups in free-floating tissue?  Do you buy a 24-well dish that can withstand 80 deg Celsius or tediously place tissue sections in falcon tubes?

blue 42
« on: January 20, 2011, 05:23:33 PM »

Offline excalibur

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Re: blue 42
« Reply #1 on: January 21, 2011, 01:15:10 PM »
Improvise. Any container that is tall enough so the water from the water bath does not spill over would work.

10, 25, 50ml beakers, coplin jars, juice glasses, tiny rubbermaid for condiments (can be found at the dollar store in packages of 4-10 and even have lids), etc.
Paula K. Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
8901 S Santa Fe, Suite G
Oklahoma City, OK 73139
405-759-3959
www.excaliburpathology.com

Re: blue 42
« Reply #1 on: January 21, 2011, 01:15:10 PM »