Author Topic: Help with automatic cell counting with ImageJ  (Read 18239 times)

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Offline laura4711

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Help with automatic cell counting with ImageJ
« on: January 24, 2011, 12:34:14 AM »
Does anybody have experience with counting cells with ImageJ? I've been trying to do automatically count cells in digital images and am having trouble.

I know that you have to convert the image to grayscale and then use the threshold before using the analyze tool to get the # of cells. My problem is that I can't get the cells outlined correctly by using the threshold tool. It seems like maybe my entire image is too close to the same color? There's not a wide variation of gray tones, I'm not sure if that matters, I don't have a lot of experience imaging.

If anybody has experience with this please let me know if you have some ideas on how to troubleshoot, thanks!

Help with automatic cell counting with ImageJ
« on: January 24, 2011, 12:34:14 AM »

Offline richard03

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FDossani

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Re: Help with automatic cell counting with ImageJ
« Reply #2 on: October 12, 2011, 01:25:50 AM »
Great..Thanks richard03. Definitely a help for us to learn...

Offline VSimon

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Re: Help with automatic cell counting with ImageJ
« Reply #3 on: December 20, 2013, 07:04:14 AM »
Hi there,

I'm trying to download the plugin they mention in the video ITCN_1_6.jar, but cant get it due to a broken link.

Does anybody have it or another link they can send to me?

Cheers,
Simon

Re: Help with automatic cell counting with ImageJ
« Reply #3 on: December 20, 2013, 07:04:14 AM »