Does anybody have experience with counting cells with ImageJ? I've been trying to do automatically count cells in digital images and am having trouble.
I know that you have to convert the image to grayscale and then use the threshold before using the analyze tool to get the # of cells. My problem is that I can't get the cells outlined correctly by using the threshold tool. It seems like maybe my entire image is too close to the same color? There's not a wide variation of gray tones, I'm not sure if that matters, I don't have a lot of experience imaging.
If anybody has experience with this please let me know if you have some ideas on how to troubleshoot, thanks!