General Research Topics > Cell Biology

Help with automatic cell counting with ImageJ

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laura4711:
Does anybody have experience with counting cells with ImageJ? I've been trying to do automatically count cells in digital images and am having trouble.

I know that you have to convert the image to grayscale and then use the threshold before using the analyze tool to get the # of cells. My problem is that I can't get the cells outlined correctly by using the threshold tool. It seems like maybe my entire image is too close to the same color? There's not a wide variation of gray tones, I'm not sure if that matters, I don't have a lot of experience imaging.

If anybody has experience with this please let me know if you have some ideas on how to troubleshoot, thanks!

richard03:
Check this video for Cell Counting using ImageJ.

http://www.youtube.com/watch?v=PqHFsmS1_JY

http://www.youtube.com/watch?v=8c68qIz_ftw&feature=related

FDossani:
Great..Thanks richard03. Definitely a help for us to learn...

VSimon:
Hi there,

I'm trying to download the plugin they mention in the video ITCN_1_6.jar, but cant get it due to a broken link.

Does anybody have it or another link they can send to me?

Cheers,
Simon

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