Author Topic: Rat endothelial cell marker  (Read 5246 times)

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Offline Amita

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Rat endothelial cell marker
« on: February 02, 2011, 06:34:03 AM »
I would appreciate your opinion on selection of
antibody for rat endothelial cell immunohistochemistry staining.
I wish to perform  IHC on paraffin section of  kidney of  SD rats
(ischemia reperfusion of renal artery model).I am confused with selection for  CD-31 or RECA-1, which will give me the better result to visualize the mature or damaged endothelial cells.

I have sort listed the santacruz sc-1506 Ab (CD-31) or abcam (RECA-1), but
not sure about its result on paraffin section. Which antibody should i select or do you suggest me to use other antibody? Your view will be greatly appreciated.
Amita
hanks, Amita dubey

Rat endothelial cell marker
« on: February 02, 2011, 06:34:03 AM »

Offline excalibur

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Re: Rat endothelial cell marker
« Reply #1 on: February 02, 2011, 11:28:53 AM »
CD31 works best on tissue fixed in a zinc formalin, but it can be done on formalin fixed paraffin embedded tissues with the use of citrate buffer antigen retrieval.
Paula K. Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
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Re: Rat endothelial cell marker
« Reply #1 on: February 02, 2011, 11:28:53 AM »