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Author Topic: IHC automation  (Read 9474 times)

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Offline Alexander Hilarov

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IHC automation
« on: February 03, 2011, 12:09:16 PM »
Dear colleagues,
I've got some questions about automation. Right now we are writing a hadbook on IHC. We would like to make an overview about automated systems.
I know well some modern stainers (ventanas, bonds, dako and labvision autostainers, intellipath); I've heared about xMatrix and Celerus.
1. Are there any other stainers?
2. Does anybody knows  any source where I may find about a history of automation in IHC? What were the first machines or other equipment to automate IHC staining? How they developed?

Thanks for any help.

IHC automation
« on: February 03, 2011, 12:09:16 PM »

Offline excalibur

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Re: IHC automation
« Reply #1 on: February 05, 2011, 12:04:18 PM »
I started doing IHC back in the 80s. All we had then were damp papertowels in a large glass petri dish and sat the slides on top of applicator sticks broken to fit the dish. The lid was used to form a humidity chamber.

For larger numbers of slides, we used plastic slides trays and boxes, tupperware, etc.

I believe the first automated IHC was by Dr. David Brigati at the Univ. of Oklahoma, who invented the capillary gap Probe-On system. It was marketed by Fisher and used a Histomatic 172 automatic stainer. In 1985, I purchased one to do H&Es. I also saw him demonstrate the first piece of equipment for insitu.

Shandon then developed the Sequenza system that used coverplates on the slides and are placed vertically in a rack with a lid to form the humidity chamber. I still use this system manually! One rack of ten slides can also be placed on the automated Cadenza.

Then came Ventana, etc.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069

Re: IHC automation
« Reply #1 on: February 05, 2011, 12:04:18 PM »