Wow had my proteins expressed and antibodies produced!!! they were phenominal!!
I am writing on here as it was after communication on this site that I out-sourced this project to a UK company. I won't name the company on here as I don't want to be a walking advertisement but they expressed a very difficult protein for me in a rapid time scale... and then produced some rabbit polyclonals for me!!
Has saved me months of work in the lab. If anyone wants to use the same service please let me know and I will give you details of where I went.
Thanks again to all for the valuable input and advice I received.