Author Topic: F4/80 staining  (Read 6300 times)

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Offline fransisca

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F4/80 staining
« on: February 11, 2011, 07:14:44 PM »
Dear All,
I'm going to do F4/80 staining on mouse brains that have been fixed in formalin at 4C for more than 24 hours (~40 hours). I know it's not supposed to be that long. However, since I don't have any more mice, do you have any advice on how to make these tissues work for F4/80 immunostaining? Any special recipe for permeabilization of this overly-fixed tissue? Thanks so much..

-Fransisca-

F4/80 staining
« on: February 11, 2011, 07:14:44 PM »

Offline richard03

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Re: F4/80 staining
« Reply #1 on: February 13, 2011, 02:03:23 PM »
40 hour fixation is not too long. With antigen retrieval, you will be able to make it to work. Refer to this protocol for more information.

http://www.ihcworld.com/_protocols/antibody_protocols/f4.80_caltag.htm

Offline inmuno

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Re: F4/80 staining
« Reply #2 on: October 31, 2013, 12:20:13 PM »
Try with proteinase k 5 minutes and after that a citrate buffer bath of five minutes.It works for my F4/80 antibody (Serotec)

hope this helps


Re: F4/80 staining
« Reply #2 on: October 31, 2013, 12:20:13 PM »