Author Topic: Tannic acid  (Read 4337 times)

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Offline pweston

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Tannic acid
« on: February 14, 2011, 01:59:30 PM »
I want to immerse my samples in tannic acid with uranyl acetate before osmicating, to enhance contrast for TEM.  When making the solution, I added the tannic acid to the uranyl acetate (4% to 2% respecively for a final concentration of 1% and 0.5% resp. when I add my sodium cacodylate buffer).  When I added the tannic to the UA I got major precipitation.  I filtered each solution as I added so I know this is bona fide ppt. and not carry over from the original solutions.  Is this what is supposed to happen?  I don't want to put my samples in a solution with all that ppt. 

Should I/can I expose seperately, i.e. UA, rinse,  then Tannic acid?  The protocols I see in papers  seem to imply doing them together.

Any help would be much appreciated,

Thanks,

Paula 

Tannic acid
« on: February 14, 2011, 01:59:30 PM »