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Author Topic: Macrophage marker on mouse heart  (Read 6992 times)

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Offline babayao

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Macrophage marker on mouse heart
« on: February 20, 2004, 02:24:04 PM »
Please help me if there anyone who has experince on IHC of macrophage marker on mouse heart tissue frozen sections?

I have done BM8, F4/80 and cd11b, they all not good. BM8 and F4/80 no reactions and cd11b was too old. Is there anybody done the CD68 or other markers on mouse heart tissue?

looking forward to any useful suggestions~

Thank you very much
ello~

Macrophage marker on mouse heart
« on: February 20, 2004, 02:24:04 PM »

Offline babayao

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Macrophage marker on mouse heart
« Reply #1 on: October 11, 2005, 09:58:06 AM »
Hi, everyone,

no one answered me?
ok, now i answer myself, the CD68 works well in the mouse heart.

good luck!
ello~

Offline ImmunoNYC

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Macrophage marker on mouse heart
« Reply #2 on: October 12, 2005, 10:56:27 AM »
I have never worked on mouse heart for macrophages - otherwise I would have answered your question - but use CD11b, F4/80 routinely on other tissues with good success. I would love to get CD68 working. Can you let me know where you bought your CD68 from and your protocol? Are you working on frozen or paraffin and what concentration and time did you use for your primary?

THANKS! :)

Offline jonnieplumb

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Macrophage marker on mouse heart
« Reply #3 on: October 14, 2005, 08:47:55 AM »
Hi Dako have a good CD68 antibody (cat no M0876) that I have used on both FFPE and SF tissue by both SABC-Px and indirect immunofluorescence.

Offline broiler25

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Macrophage marker on mouse heart
« Reply #4 on: October 14, 2005, 12:04:47 PM »
Rat anti-mouse CD68 clone FA-11 from Serotec works fantastically well.  Proteinase K pretreatment is required (hier doesn't work).

Macrophage marker on mouse heart
« Reply #4 on: October 14, 2005, 12:04:47 PM »