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Author Topic: Hier and tissue architecture  (Read 4746 times)

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Offline alexandrabetts

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Hier and tissue architecture
« on: May 31, 2004, 02:37:30 PM »
I'm carrying out immunohistochemistry (several antibodies) on fibrous lesions. When using HIER I'm getting almost total loss of tissue architecture and tissue detachment from the slides both when using pressure cooker and microwave (citrate buffer pH6). Doubling the concentration of APES adhesive helped reduce loss of adhesion but there was still loss of architecture.
I've tried using citrate buffer pH 3 at 37C but that didn't work.
Any ideas?

Sandra
andra Betts

Hier and tissue architecture
« on: May 31, 2004, 02:37:30 PM »

Offline richard03

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Hier and tissue architecture
« Reply #1 on: June 03, 2004, 03:51:10 PM »
Sandra,

I am suggesting to use a little bit of gelatin in your water bath to solve the problem. If it didn't work, try to coat slides with gelatin solution

Recipe for Chrome Alum Gelatin Solution:
Gelatin, type A, 275 Bloom -------------------- 3 g
Chromium potassium sulfate ------------------ 0.5 g
Distilled water ----------------------------------- 1000 ml

Heat to 60 C to dissolve gelatin and then add chromium potassium sulfate.

Hope this helps.

Richard

Hier and tissue architecture
« Reply #1 on: June 03, 2004, 03:51:10 PM »