Abcam Ad

Author Topic: Frozen Sections  (Read 4832 times)

0 Members and 1 Guest are viewing this topic.

Offline Jtess

  • Newbie
  • *
  • Posts: 1
Frozen Sections
« on: May 07, 2004, 11:12:47 AM »
I am using tissue that was fixed for 4 hours in 4% PFA and then eventually embedded in OCT and frozen.  Does an antigen retrieval step need to be done?

Frozen Sections
« on: May 07, 2004, 11:12:47 AM »

Offline richard03

  • Administrator
  • GoldMember
  • *****
  • Posts: 1212
Frozen Sections
« Reply #1 on: May 07, 2004, 12:51:04 PM »
The vast majority of Antigen Retrieval are done on formalin-fixed, paraffin-embedded tissue sections. Fresh frozen sections are not needed for antigen retrieval. However, some (very few) PFA fixed frozen tissue sections are needed for antigen retrieval depending on the antibodies used. I suggest do a test stain without AR. If it worked, that's it.

Richard

Frozen Sections
« Reply #1 on: May 07, 2004, 12:51:04 PM »