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Author Topic: Cell Line Staining  (Read 1922 times)

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Offline Jason

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Cell Line Staining
« on: July 20, 2004, 01:00:56 PM »
Does anyone know of a dependable H&E and/or IHC-DAB staining protocol for cell lines that are heat-fixed to adhesive slides?

-Jason

Cell Line Staining
« on: July 20, 2004, 01:00:56 PM »

Offline Tony

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Cell Line Staining
« Reply #1 on: July 21, 2004, 12:21:41 PM »
Hi Jason,

I use "Histogel" from Richard Allen Scientific to cover cell pellet and then treat this cell pellet as tissue to process for paraffin embedding and H&E, immunostaining, etc. It works beautifully.

You may want to give it a try.

Tony

Cell Line Staining
« Reply #1 on: July 21, 2004, 12:21:41 PM »