Has anyone figured out how to stain Desmin (sc-7559), Met (sc-161) and/or VEGF (sc-152) on paraffin sections of mouse tissue?
Though Santa Cruz claimed these three are okay for (formalin fixed) paraffin sections, I have never managed to see any signal. Antigen retrieval methods including BD retrievagen A or 10 mM Sodium Citrate, pH 6 in steam cooker for 15min or in combination with trypsin treatment of 60 seconds at 37 centigrade degree. Tech support contacted, a nice guy of no further help did not tell anything more than I have also known.
I have a bunch of blocks already fixed in formalin. No room to play with fixation method now.
Any suggestions? Thanks
