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Author Topic: fluorescence leaking  (Read 3364 times)

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Offline lilian howard zhou

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fluorescence leaking
« on: August 11, 2004, 12:35:39 PM »
Hi,

I am a beginner in using confocal microscope. I use PI to dye the nucleus and GFP fusion protein to study the cellular localization. But somehow the green fluorescence is too strong that it also shows speckles in red channel part. I don't know how to avoid this and get the red thing which only shows nucleus. Could anyone give me some help? Thanks!

fluorescence leaking
« on: August 11, 2004, 12:35:39 PM »