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Author Topic: Reccomend basic/good immunohistochemistry manual/handbook  (Read 3896 times)

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Offline vcolomer

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Reccomend basic/good immunohistochemistry manual/handbook
« on: March 14, 2011, 11:12:26 PM »
I need a manual that walks you through the advantages and disadvantages of the different methods to prepare, embed, immunostain, counterstain mouse brain slices.  I would prefer slices 8uM thick, to immunostained with antibodies  that requires antigen presentation, and counterstaining with hematoxylin or eosin
Thank you
Veronica

Reccomend basic/good immunohistochemistry manual/handbook
« on: March 14, 2011, 11:12:26 PM »

Offline excalibur

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Re: Reccomend basic/good immunohistochemistry manual/handbook
« Reply #1 on: March 14, 2011, 11:17:36 PM »
There is no simple handbook. There are many variables involved with the choice of antibodies.

I suggest you start with a histotechnique book such as Carson's or Sheehan/Hrapchak.
Paula Keene Pierce, HTL(ASCP)HT
Excalibur Pathology, Inc.
5830 N Blue Lake Dr.
Norman, OK 73069
405-759-3959
www.excaliburpathology.com

Re: Reccomend basic/good immunohistochemistry manual/handbook
« Reply #1 on: March 14, 2011, 11:17:36 PM »