Antibody-Based Technologies > Antibody W

WT-1 and CD138

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sergio:
I would like to known what the better clone and protocol for WT-1 and CD138.
Thanks a lot
Sincerely,

SÚrgio Romano.

richard03:
Here is a WT-1 protocol and it works well on formalin-fixed, paraffin-embedded tissues.

http://www.ihcworld.com/_protocols/antibody_protocols/wt1_santa_cruz.htm

Richard

anthony wing:
hi sergio,

i use a dako product for WT1(monoclonal)-cut  fresh slide
                                                            -pepsin 10 mins
   
and for cd138 dako product monoclonal- microwave citrate buffer ph6.0
                                                            using a TTmega
       

for both antibodies i use ID labs link  and labell peroxidase
system.
       
if you have any more questions let me know

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