Thank you for your kind advice, I have tried and it worked. Now I am performing TUNEL assay on cell culture, I have problem with methyl green counter staining, after 30 seconds of counter staining the stain was very dark, I think my TUNEL is okay because I can see some apoptosis cells, how can I reduce the counter staining color and save my slides?
Any advice will be very appreciate
Regards
Gus