Author Topic: Caspases (Read 8645 times)

ljaw100 · « **on:** September 07, 2004, 07:13:34 AM »

Is anyone else using activated caspases to try and detect apoptosis in tissues either parrfin or GMA? I'm having a few problems and would be grateful of any help.

Thanks

Laura

« **on:** September 07, 2004, 07:13:34 AM »

brian · « **Reply #1 on:** September 08, 2004, 09:56:09 PM »

I have been using active caspase3 from R&D Systems with good success by refering to this protocol:
http://www.ihcworld.com/_protocols/antibody_protocols/caspase3_r&d_system.htm

Brian

ImmunoNYC · « **Reply #2 on:** July 07, 2005, 12:50:39 AM »

I use cleaved caspase 3 from Cell Signaling with great success. What problems are you having, I would be glad to help.

vivfly · « **Reply #3 on:** March 08, 2006, 09:00:01 AM »

Quote from: "MaximinaNYC"

I use cleaved caspase 3 from Cell Signaling with great success. What problems are you having, I would be glad to help.

hi, maximina, i noticed that most of these caspase3 antibodies are appied for paraffin-embedded slides. do you have any idea whether i could use them for frozen sections?thanks

« **Reply #3 on:** March 08, 2006, 09:00:01 AM »

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Author Topic: Caspases (Read 8645 times)