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Double labeling with antibodies raised in same species

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amiryala:
I need to do double labeling with antibodies raised in mouse (no other host species are available)
-I am trying with one biotynlated primary
Any ideas how I could make it work.
 :shock: thank you !

richard03:
There is recently published paper about double labeling using identical species-derived primary antibodies. Here is the link to the paper.

http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15314088

Hope this helps.

Richard

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