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Author Topic: HEIR with citrate buffer pH3, 37 degrees  (Read 7181 times)

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Offline Furay

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HEIR with citrate buffer pH3, 37 degrees
« on: September 22, 2004, 09:02:34 PM »
Has anyone had any experience with using the following method for antigen retrieval:

10mM Citrate Buffer pH3.0 Antigen Retrieval.
http://www.chemicon.com/techsupp/citratebuffer.asp

I was looking at using this method as opposed to the standard 95-100 degree, pH6 Citrate buffer retrieval method (as this high heat method causes my samples to "boil" off the slides).

Thanks,

Furay

HEIR with citrate buffer pH3, 37 degrees
« on: September 22, 2004, 09:02:34 PM »

Offline brian

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HEIR with citrate buffer pH3, 37 degrees
« Reply #1 on: September 30, 2004, 09:22:40 AM »
This is not commonly used buffer for antigen retrieval. However, some antigens need to be retrieval using low pH buffer at 37 C. For example, Beta-Amyloid proteins, BrdU, etc. So I don't think this buffer can be used to replace Citrate Buffer (pH6.0) universally.

Brian

HEIR with citrate buffer pH3, 37 degrees
« Reply #1 on: September 30, 2004, 09:22:40 AM »