Thank you for the responses.
I am sectioning fat to see difference between heat-treated and untreated fat. I do have to section frozen adipose tissue (and have successfully done it, but it has to be 50 um thick).
I would really like to be able to stain the lipids confined within the fat cell, especially for the untreated fat block. For now, I am going to try to fix the tissue first in either 10% formalin or 4% PFA then cryoprotect in 30% sucrose before embedding to see if that will prevent disruption of cell membranes.
I would eventually try to use this protocol for staining/locating lipid in other tissue (which would be cut frozen about 30um thick). For now, I'm trying to optimize