Author Topic: do perm steps wash away extracellular collagen?  (Read 3366 times)

0 Members and 1 Guest are viewing this topic.

Offline NLi

  • Newbie
  • *
  • Posts: 1
do perm steps wash away extracellular collagen?
« on: May 18, 2011, 02:44:21 PM »
Hi,

I'm doing icc staining on intracellular collagen I in human fibroblast cell cultures. I used 0.5% PBST (x 15 mins) to permealize the cells.

If there're extracellular collagen on the cell surface, do anyone know if this concentration of PBST will disrupt the extracellular collagen resulting in it being washed away?

Thanks for any advise.


do perm steps wash away extracellular collagen?
« on: May 18, 2011, 02:44:21 PM »