Author Topic: Preabsorption in western blotting  (Read 4288 times)

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Offline rajamani

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Preabsorption in western blotting
« on: July 01, 2011, 01:00:33 PM »
I have been working with membrane protein obtained from brain tissues.  I need to validate a specificity of an antibody.  Antibody gives a single strong band with all tissues.  i would like to do preabsorption with the same.  I have used two preabsorption ratios, 5ug of ab to 125ug of ag and 10ug of ab to 125ug of ag.  I could block the band with 10ug of ab.  Does this sound right and logical?

thanks

Preabsorption in western blotting
« on: July 01, 2011, 01:00:33 PM »