Author Topic: Hypoxic Detection w/ Anti-Heat Shock Proteins  (Read 6545 times)

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Offline schao

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Hypoxic Detection w/ Anti-Heat Shock Proteins
« on: October 06, 2004, 04:20:54 PM »
Hi,

I'm interested in marking hypoxic areas in murine tissue and tumor samples for use in either IHC or fluorescence microscopy. I've read about methods using pimonidazole adducts. However, my PI is more interested in antibodies that track hsp's. Since there are a myriad of hsp's, I'm not really sure where to begin. Does anyone have any advice?

Susan

Hypoxic Detection w/ Anti-Heat Shock Proteins
« on: October 06, 2004, 04:20:54 PM »

Offline richard03

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Hypoxic Detection w/ Anti-Heat Shock Proteins
« Reply #1 on: October 06, 2004, 08:33:45 PM »
HIF-1 alpha (Hypoxia Induced Factor) is a good marker for Hypoxic tumors. Additional along the hypoxia pathway, there are some other markers such as CA-IX and Heme-Oxygenase-1 (HO-1).

Richard

Offline richard03

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Re: Hypoxic Detection w/ Anti-Heat Shock Proteins
« Reply #2 on: September 27, 2007, 07:38:29 PM »
There are many more hypoxia markers you can choose from depending on your specific research interest.

http://www.antibodybeyond.com/reviews/tumor-markers/hypoxia-marker.htm

Re: Hypoxic Detection w/ Anti-Heat Shock Proteins
« Reply #2 on: September 27, 2007, 07:38:29 PM »