It's been a million years since I last used a cryostat and for the life of me I can't remember how to deal with my slides while sectioning.
I'm cutting fresh frozen tissue that is intended to go straight back into the -80 freezer as soon as the sectioning is completed (no fixation). Is it ok to temporarily (minutes, not hours) keep the slide at room temperature with sections already on it while I cut the next section(s)? This obviously means that the already cut sections will be thawing and beginning to dry before the sectioning is completed and the slide is eventually thrown back into the -80.
The plan is to have 3 sections each from 6 different blocks on each slide. Should I keep the slide at RT while collecting the 3 sections, then back into the cryostat while I set up the next block, then back to RT while sectioning, etc.? Or am I being too paranoid and leaving them to thaw and dry at room temperature before going back into the -80 is standard protocol?
Thanks a bunch!